MONOCARBOX TRANS 4 (HU); RBX-50UG

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-Monocarboxylate Transporter 4 Antibody detects level of Monocarboxylate Transporter 4 & has been published & validated for use in ELISA & WB.

Western blot: 1-5 µg/mL using ECL.
Immunohistochemistry: Not tested. Recommended antibody testing dilution: 5-10 µg/mL on paraformaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG219) per well.
Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 19 amino acid peptide sequence within the cytoplasmic domain #4 of human MCT4 (Wilson et al., 1998; Price et al., 1998).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB3548P

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Specificity

Recognizes human Monocarboxylate Transporter 4 (MCT4). The immunogen shows no significant sequence homology with other MCT. MCT4 is prominently expressed in skeletal muscle and other cells with high glycolytic rate such as tumors cells and white blood cells suggesting an important role in lactic acid efflux.

Note: According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Consult Halestrap and Price (1999) Biochem. J. 343:281-299.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.